BIOCHEMICAL EVALUATION OF COMPENSATORY RENAL HYPERTROPHY

II. Effects of Renotrophic Factor(s) on Protein Synthesis at Polysomal Fraction

Abstract

The present study was undertaken to clarify whether or not and if affirmative how the renotrophic factor(s) which is postulated to initiated compensatory renal hypertrophy demonstrates influence on protein synthesis at the polysomal fraction. Serum taken from rats 48 hours after unilateral nephrectomy, when compared to serum from sham operated animals, stimulated the incorporation of 1-leucine-¹⁴C into the free polysomal fraction from rat kidneys. The increment of the incorporation was dose-dependent of the serum administered to the subcellular protein synthesis system. The increase of the incorporation of 1-leucine-¹⁴C was not noticed at the bound polysomal fraction of rat kidneys.
Growth hormone (bovine), which is postulated to be identical with renotrophin(s), did not demonstrate any influence on the incorporation of 1-leucine-¹⁴C into both free and bound polysomal fractions of rat kidneys. Addition of cyclic AMP with bovine growth hormone did not stimulate the incorporation of 1-leucine-¹⁴C into both polysomal fractions of rat kidneys. It is inferred that renotrophin(s) has a stimulatory effect on the process of protein synthesis.