ESTABLISHMENT OF A NEW CELL LINE (NRC-12) DERIVED FROM A HUMAN RENAL CELL CARCINOMA AND ITS CHARACTERISTICS

Abstract

A permanent established cell line (NRC-12) derived from a human renal cell carcinoma is described. These cells have been maintained for 30 months and over 140 in vitro passages. The growth of these epithelial cells was characterized by the absence of contact inhibition and the doubling time was constant at 48 hours. Electron microscopic study showed the localized nucleus and prominent microvilli resembling the brush border-like structures of the renal cell carcinoma on the opposite side of the nucleus. Histrogical examination of the heterotransplant into the cheek pouches of a golden hamster verified that the nature of the cells was identical to clear cell adenocarcinoma, resembling the original tumor of the patient. Chromosomal analysis revealed the presence of two hypotetraploid stemlines (86 and 88) and a secondary constriction on chromosome A3 of all cells.