ESTABLISHMENT OF A NEW CELL LINE (NBT-2) DERIVED FROM A HUMAN URINARY BLADDER CARCINOMA AND ITS CHARACTERISTICS
1979 Volume 70 Issue 3 Pages 351-357
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1979 Volume 70 Issue 3 Pages 351-357
A permanent established cell line (NBT-2) derived from a human urinary bladder carcinoma is described.
The tissue for cell cultures was taken from a 64-year-old male during partial cystectomy for urinary bladder carcinoma. A papillary growth, 1.0×1.5×1.5cm in size was found in the vicinity of the left ureteral orifice with basal invasions into the bladder wall. According to the pathological report, though the papillary growth appeared to be of grade III transitional cell carcinoma, the intramuscular invasions were proved to be more undifferentiated (grade IV).
Those cultured cells have been maintained for 28 months and passed 120 times. The bases that NBT-2 cells are identical with those of the urinary bladder carcinoma are as follows:
1) The tissue for cell cultures was removed from the urinary bladder carcinoma.
2) The epithelial-like cells grew more rapidly than the fibroblast-like cells and the final transition in primary cultures showed a complete monolayer of epithelial-like cells.
3) Ultrastructural characteristics of NBT-2 cells resembled those of high grade urinary bladder carcinoma cells.
4) Chromosomal analysis of the early cultured cells (4th in vitro passages) showed the presence of two hypertriploid stemlines (72 and 74).
5) Histological examination of the heterotransplant into the cheek pouches of a golden Syrian hamster revealed that the nature of the cells was much alike to undifferentiated transitional cell carcinoma (grade IV) of the original tumor.
