1981 Volume 72 Issue 2 Pages 178-184
The DNA content of exfoliative single cells from bladder washings in patients with bladder cancer has been analysed by means of flow cytometry. Materials were obtained by bladder irrigation and preparation was made by the cell isolation with trypsin and the DNA staining with propidium iodide.
The DNA histograms in 37 cases from 39 cases with bladder cancer revealed double or more peaks consisted of 2c (diploid) fraction and hyperploid fractions, meanwhile histograms in all 11 cases with benign diseases revealed only single peak of 2c fraction. The hyperploid peaks on DNA histograms disappeared in 9 cases from 10 cases with inactive bladder cancer after TUR-Bt. Agreement of these results with histological findings were superior to that of cytological findings with histological findings in the same materials.
We classified the DNA histograms into four grades by the original criteria. There appeared to be a correlation of DNA histogram grading with histological grading and staging. It is suggested that automation of cytological examination might be possible by application of flow cytometry.
