ELECTRON MICROSCOPIC STUDIES OF IN VITRO CULTIVATED HUMAN BLADDER CELL LINES
KK-47, KW-103 AND HCV-29
1982 Volume 73 Issue 3 Pages 294-307
Details
1982 Volume 73 Issue 3 Pages 294-307
Electron microscopic studies were carried out on the 3 established cell lines; KK-47 and KW-103 were derived from transitional cell carcinomas of human urinary bladder and HCV-29 was originated from the benign-appearing transitional epithelium of a patient treated for a bladder tumor by irradiation.
Pellets of KK-47 cells grown in log growth phase at the 40, 120 and 200th passage levels were subjected to transmission electron microscopic studies. The cells at the 120th passage level were also studied by a scanning electron microscope immediately after subcultivation and 6, 12, 18, 24, 60 and 120 hours later. Concerning KW-103 and HCV-29, their colonies grown on Petri dishes 14 days after cell seeding were utilized for transmission electron microscopic studies.
The results obtained were as follows:
1. KK-47 and KW-103 cells closely resembled each other with respect to the transmission electron microscopic appearances. Round or oval nuclei with many protrusions, numerous microvilli, plentiful free ribosomes and desmosome-like junctional complexes between adjacent cells were observed in cells of both lines.
2. Well-developed rough endoplasmic reticulums and bundles of intracellular filament under the plasma membrane were observed in HCV-29 cells.
3. An increase of cell organella was observed in KK-47 cells at the 200th passage level compared to the earlier passage levels.
4. The scanning electron microscopic findings of KK-47 cells were characterized by a loss of contact inhibition as evidenced by piling-up, random arrangement and colony formation.
From these findings it was suggested that KK-47 and KW-103 cells had several morphological characteristics of transitional epithelial cells, the motility of HCV-29 cells was less than that of the other 2 cell lines as indicated by the presence of bundles of intracellular filament under the plasma membrane, and KK-47 cells were transformed during a long-term cultivation.
