STUDY OF IN VITRO SENSITIVITY OF HUMAN BLADDER CANCER CELL LINES TO SEX STEROID HORMONES
Combined Effects of Adriamycin or Mitomycin-C and Estradiol-17β on T24 Cells
1982 Volume 73 Issue 7 Pages 862-867
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1982 Volume 73 Issue 7 Pages 862-867
In vitro sensitivity of the established cell line from human urinary bladder cancer, T24 and 253J, to various sex steroid hormones have been examined by the colony formation method, and specific cytotoxic effect of estradiol-17β (E2) was reported.
In this study, the combined effects of Adriamycin (ADM) or Mitomycin-C (MMC) and E2 on the T24 cells were examined by the colony formation method. There was no significant difference between cytotoxic effects of ADM or MMC alone and those in combination with E2 when exposed for 2 hours. However, when exposed for 24 hours, ADM or MMC in combination with E2 showed significantly more cytotoxic effects than ADM or MMC alone at low concentrations (≤10-3μg/ml) of ADM or MMC (p<0.05-p<0.001).
