EFFECT OF REDUCED GLUTATHIONE ON N-BUTYL-N-(4-HYDROXYBUTYL)-NITROSAMINE-INDUCED URINARY BLADDER CANCER IN RATS
1983 Volume 74 Issue 12 Pages 2077-2080
Details
1983 Volume 74 Issue 12 Pages 2077-2080
Effect of reduced glutathione (GSH) on N-butyl-N-(4-hydroxybutly) nitrosamine (BHBN)-induced urinary bladder cancer in rats was studied. F344 male rats at the age of 6 weeks old were used. BHBN was dissolved in the drinking water at a concentration of 0.05%. GSH was mixed with poweder diet at a concentration of 0.5%. Rats were treated with or without BHBN for 6 weeks from the 4th experimental day, and were also treated with or without GSH from the start of experiments to the 3d day after the end of BHBN treatment. All the animals were killed 40 weeks after the start of BHBN treatment.
The incidences of papilloma and carcinoma were 60% (6/10) and 20% (2/10) respectively in the group treated with BHBN and GSH, and 47% (7/15) and 7% (1/15) in the group treated with BHBN alone. There was no statistically significant difference between these two groups.
In this experment, GSH failed to inhibit BHBN-induced bladder carcinogenesis in rats. Application of GSH at higher doses or during promotion step of BHBN-carcinogenesis appears important.
