DETECTION OF BLOOD-GROUP ANTIGENS IN URINARY BLADDER CANCER TISSUE BY PEROXIDASE-ANTIPEROXIDASE METHOD

Abstract

Recongnition of the innate aggressiveness of a bladder cancer early in the course of the disease would greatly facilitate management, and potentially idenitify those tumors which are likely to recur and invade.
Recently, several investigators have reported on the significance of the detection of blood-group ABO(H) antigens on the surface of bladder tumor of the Specific Red Cell Adherence (SRCA) test, This test, in many cases, proved to be a valuable tool for predicting the behaviour of non-invasive tumors.
However, the SRCA test has limitation. First, the procedure does not reliably detect the O(H) antigen. Furthermore, it is very difficult to locate the antigen in tissue by the SRCA test because the absorbed red blood cells covered morphological details of the specimen.
In this study, the Peroxidase-antioperoxidase (PAP) method was employed to detect the antigens in 23 cases of human bladder cancer. The PAP method is more sensitive and easier to perform than the SRCA technique, and displays the PAP reaction and histopathology of the tissue on the same slide. And the PAP staining could show the exact location of the antigen in reference to well preserved tissue structures.
The PAP method was estimated to be more suitable than the SRCA test for detection of ABO(H) antigens in human bladder tumors.