STUDY ON IN VITRO SENSITIVITY OF HUMAN BLADDER CANCER CELL LINES TO VARIOUS SEX STEROID HORMONES
Effect of Estradiol-17β on T24 Cells and 253J Cells
1983 Volume 74 Issue 6 Pages 897-906
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1983 Volume 74 Issue 6 Pages 897-906
Previously, in vitro sensitivity of the established cell line from human urinary bladder cancer, T24 and 253J, to various sex steroid hormones was examined by the colony formation method, and specific cytotoxic effect of estradiol-17β (E2) was reported.
In this study, the effect of E2 on the biological and morphological characteristics of both cells were examined, and the mechanism of specific cytotoxic effect of E2 on both cells were analyzed. Human breast cancer cells, HBC4, and human uterine cancer cells, OG, were used as the control.
The results were as follows:
1) The growth of T24 and 253J cells was suppressed by E2 at concentrations of more than 5μg/ml.
2) Doubling time of T24 and 253J cells increased with increasing concentration of E2.
3) The mitotic index of T24 and 253J cells decreased with increasing concentration of E2.
4) When exposed to E2 for 6 hours or more, the morphology of T24 and 253J cells were affected by E2 at concentrations of more than 5μg/ml.
5) In the colony formation method, the significant cytotoxic effect could be observed in T24, 253J and HBC4 cells by E2, and in HBC4 cells by progesterone.
6) Correlation between the effect of E2 on the plating efficiency of four cell lines and the labeling index of 3H-E2 of four cell lines could be recognized.
7) The specific cytotoxic effect could be demonstrated in the S phase of T24 cells with the use of E2.
