1983 Volume 74 Issue 6 Pages 945-955
Serum prostatic acid phosphatase (PAPase) activity and the ratio of PAPase activity against the total acid phosphatase (APase) activity were determined by the immunochemical method, previously reported in our first, second and third reports, in 16 patients with prostatic cancer, 18 patients with benign prostatic hypertrophy, 11 patients with cancer of other organs (3 patients with renal cell cancer, 3 patients with bladder cancer and 5 patients with liver cancer) and normal subjects of both sexes.
The results were compared with those determined by the conventional method and radioimmuno assay (RIA).
1) Total APase activity, PAPase activity inhibited by L-tartrate, PAPase activity determined by the immunochemical method and the ratio of it against total APase activity and PAPase determined by RIA were with the normal range in normal subjects of both sexes and in patients with cancer of organs other than the prostate, and no false positive cases were observed.
2) One of the 18 patients with benign prostatic hypertrophy showed false positive results in total APase activity and the ratio of PAPase activity determined by immunochemical method against total APase activity.
PAPase activity inhibited by L-tartrate and those determind by the immunochemical method were within the normal range in all patients with benign prostatic hypertrophy, while PAPase determinned by RIA exhibited false positive result in 2 of 18 patients.
3) When the relationship between PAPase activity and the extent of infiltration was studied in patients with prostatic cancer, total APase activity and PAPase activity inhibited by L-tartrate were within the normal range in all 7 cases of stage T1, T2 group and positive in 5 of 9 cases of stage T3, T4 group.
PAPase activity determind by the immunochemical method was positive in 2 of 7 cases of stage T1, T2 and in 8 of 9 cases of stage T3, T4.
The ratio of PAPase determind by the immunochemical method against APase activity was positive in 3 of 7 cases of stage T1, T2 and in all 9 cases of stage T3, T4.
On the other hand, PAPase determind by RIA was positive in one of 7 cases of stage T1, T2 and in 7 of 9 cases of stage T3, T4.
4) These results suggest that PAPase activity determind by the immunochemical method may be as useful as that determind by RIA for the diagnosis of prostatic cancer.
