SENSITIVITY TESTS OF ANTICANCER DRUGS WITH HUMAN TUMOR STEM CELL ASSAY
1. Primary Culture of Human Renal Cell Carcinoma in Soft Agar Medium
1983 Volume 74 Issue 7 Pages 1081-1085
Details
1983 Volume 74 Issue 7 Pages 1081-1085
In order to investigate the chemosensitivity test with a stem cell assay for renal cell carcinoma, primary culture of human renal cell carcinoma in soft agar was studied.
The soft agar culture was constructed with Ham's F-12 medium, 8% fetal calf serum and agarose. Final agarose concentrations were 0.4% for the basal layer and 0.24% for the culture layer. And 0.2% collagenase was used for isolating the cancer cells. Dissociated cancer cells were plated into the soft asar dish and cultured for 3 to 4 weeks. Formed colonies (more than 30 cells per colony) were counted.
Colony-formation in the soft agar dish was observed in 13 out of 16 cases (81%) and these plating efficiency were 0.2 to 0.5%.
Preliminary chemosensitivity tests were performed using our systems. The results indicated that this stem cell assay was applicable to clinical cancer chemotherapy.
