STUDIES OF RICE-BRAN THERAPY FOR CALCIUM UROLOTHIASIS WITH IDIOPATHIC HYPERCALCIURIA
II. In vitro Experiments on Binding of Calcium and Rice-bran
1984 Volume 75 Issue 1 Pages 10-15
Details
1984 Volume 75 Issue 1 Pages 10-15
Binding of calcium by rice-bran was investigated in vitro, for the purpose of studying the mechanism of rice-bran binding to dietary calcium in the intestines.
The following results were obtained:
1) Much of the calcium was bound by 0.1% Na-phytate (Sigma Co.). When phytin from rice-bran (0.1%), defatted rice-bran (1%) and rice-bran pharmacopoeia (1%) were dissolved with 0.1 N HCl, similar effects wre observed.
2) Phytin and defatted rice-bran were poorly soluble in neutral solutions, and high abilities to bind calcium were obtained on dissolving with 0.1 N HCl or human gastric juice.
3) Rice-bran pharm. showed a stable ability to bind calcium both in neutral and acid solutions. Phytin in rice-bran might be denatured to a highly soluble phytate by cultivation of defatted rice-bran.
4) The quantity of calcium binding of rice-bran pharm. was far greater than that of wheat-bran pharm. This has been confirmed in the reported clinical results.
5) The binding of calcium by phytic acid was increased in a weak alkaline solution.
6) The effects of rice-bran pharm. on calcium was depended on its concentration.
7) Cellulose from rice-bran did not show any abilities to bind calcium, and dephytinized rice-bran showed less than defatted rice-bran. The results suggested that phytin in rice-bran was the major factor of calcium binding.
8) Rice-bran pharm., culture products of defatted rice-bran, should be a suitable agent for rice-bran therapy in this experiment.
