TREATMENT OF ESTABLISHED HUMAN RENAL CELL CARCINOMA HETEROTRANSPLANTED IN NUDE MICE
2. Experimental Chemosensitivity of Anticancer agents
1984 Volume 75 Issue 12 Pages 1911-1920
Details
1984 Volume 75 Issue 12 Pages 1911-1920
A study was conducted to evaluate the chemotherapeutic effects of anticancer drugs against a human renal cell carcinoma obtained from Jikei University Hospital and serially passaged in nude mice.
Four chemotherapeutic agents that have shown some degree of activity against human renal cell carcinoma in the clinic and therefore were evaluated in this study were adriamycin (ADM), vinblastine (VBL), VP-16 and 5-fluorouracil (5-FU). Comparisons of the effect were made among groups of mice or nude mice given LD50, 1/2LD50 and 1/4LD50 doses.
In order to evaluate the effects of anticancer agents, statistical and histopathological evaluation were made. The two-dimensional tumor measurements taken periodically were transformed into estimates of tumor weights using the formula.
Histopathological changes of treated mice bearing renal cell carcinoma were estimated by the histopathological degenalations which have been proposed by National Cancer Research Institute (Shimosato's classification). Histopathological criteria for evaluation of therapeutic effects were classified into grades I to IV.
Significant effects of single agent therapy based on statistical evaluation were obtained with ADM (1/2LD50) and VBL (LD50) against JRC11 strains, and also were obtained with 5-FU (1/4LD50) against JRC9 and JRC11 strains. Significant effects of combination chemotherapy based on statistical evaluation were obtained with VBL (1/4LD50) and 5-FU (1/4LD50) against JRC9 and JRC11 strains. But significant effects based on histopathological evaluation were only obtained with VBL (1/4LD50) and 5-FU (1/4LD50) against JRC11 strains. And other strains which showed significant effects based on statistical evaluation disclosed hardly any histopathological chainges.
The results suggested that there was no correlation between the results obtained by statistical evaluation and those by histopathological evaluation.
