1985 Volume 76 Issue 1 Pages 96-103
Effect of antineoplastic agents on cell cycle traverse was investigated using flow cytometry and cultured cell line (OUR-10) originated from human renal cell carcinoma.
Relative DNA contents of tumor cells were determined by flow cytometry and changes of DNA histogram were studied, 24 and 48 hours after 2-hour or continuous exposure of cultured cell line to antineoplastic agents.
Mitomycin C (MMC) led to an accumulation of cells in S and G2M phase. At concentrations which inhibited cell growth moderately, the rate of cell cycle progression was delayed and cells were blocked in G2 phase. At higher concentrations, MMC inhibited the progression of cell cycle strongly.
Adriamycin (ADM) resulted in a block in G2 phase but no accumulation in S phase was observed. At higher concentrations, ADM inhibited cell cycle progression completely.
Bleomycin (BLM) showed no remarkable change of DNA histogram patterns. This was considered to be due to the cytotoxic effect of BLM on tumor cells in G1 phase.
Exposure of cultured cells to vinblastine (VBL) resulted in an accumulation in G2M phase, which was consistent with metaphase arrest of VBL.
Accumulation of cells in S and G2M phases was observed in the treatment with cis-dichlorodiamine-platinum (CDDP). At concentrations which inhibited cell growth strongly, cells were blocked in G2 phase after the slow progression through S phase. At higher concentrations, the progression of cell cycle was inhibited completely.
