1985 Volume 76 Issue 10 Pages 1499-1503
The glycerinated muscle was prepared from the rabbit's external urethral sphincter and analysed by O'Farrell's two-dimensional electrophoresis. The majority of myosin light chains (MLC) was the fast type. Crude myosins were prepared from the external urethral sphincter, the psoas and the soleus muscles. Peptide maps of myosin heavy chains (MHC) were made using S. aureus V8 protease and papain. The pattern of peptide map of MHC of the external urethral sphincter muscle was different from that of the soleus (slow twitch muscle) and quite similar to that of the psoas (fast twitch muscle) in both S. aureus V8 protease and papain digestions. Therefore both MLC and MHC of the external urethral sphincter muscle were concluded as the fast type.
