HOST DEFENSE MECHANISM IN PYELONEPHRITIS
Activation of Peritoneal Macrophage and Its Protective Effect in the Rat with Retrograde Proteus Mirabilis Pyelonephritis
1985 Volume 76 Issue 12 Pages 1869-1879
Details
1985 Volume 76 Issue 12 Pages 1869-1879
Inorder to clarify the role of macrophages (Mφ) in the host defense mechanism in rats with P. mirabilis pyelonephritis, the superoxide (O2-)-releasing activity of peritoneal Mφ, the severity of the pyelonephritis and the rate of fatal infection were examined under various conditions which modified the host defense mechanism.
For modification of the host defense mechanism, OK-432 (50KE/kg), cyclophosphamide (100mg/kg), diethylstilbestrol (200mg/kg) and carrageenan (200mg/kg) were used as a nonpsecific immunoactivator, nonspecific immunosuppressor, activator of Mφ and suppressor of Mφ, respectively.
The results were as follows:
1) The O2--relesing activity of peritoneal Mφ 7 days after the inoculation of P. mirabilis into the bladder was definitely higher than that of normal rats.
2) The O2--releasing activity of peritoneal Mφ was definitely or relatively increased when the activator of Mφ (diethylstilbestrol) or the immunoactivator (OK432) was used, but definitely decreased when the immunosuppressor (cyclophosphamide) or suppressor of Mφ (carrageenan) was used.
However, the severity of pyelonephritis and fatal infection rate were decreased only when the OK432 was used, and there was no difference in the items between the controls and the rats in which cyclophosphamide, dietylstilbestrol or carrageenan was used.
These results suggest that Mφ are activated by infection with P. mirabilis but that they play an adjunctive role in protection against infection.
