INEFECTIOUS MECHANISM IN EXPERIMENTAL CYSTITIS OF MICE

II. Influence of Endotoxin

Abstract

E. coli (06) endotoxin was inoculated into mouse bladder by transurethral technique. Histological studies were performed in mucin layer in mucosal epithelium of urinary bladder. And combined use of the endotoxin and E. coli in infectious crisis futher accelerated the process of infectious crisis.
The results obtained were as follows:
1 Endotoxin (06) 10μg/ml and 1mg/ml were inoculated into mouse bladder, and mucin layer in the mucosal epithelium and histological findings of urinary bladder were studied 2, 6, 24, and 72 hours after endotoxin inoculation.
(1) Histological findings in urinary bladder after inoculation of endotoxin 10μg/ml (50μl. PBS). Disappearance of mucin layer was not obtained. Submucosal edema of urimary bladder was manifest 2 hours after endotoxin inoculation in 40% cases and in 80% after 6 hours. But its edema was no obtained 24 and 72 hours after inoculation of endotoxin.
(2) Histological findings in urinary bladder after inoculation of endotoxin 1mg/ml (50μl. PBS). Disappearance of mucin layer was not obtained. Submucosal edema of urinary bladder was manifest 2 hours after inoculation of endotoxin in 100% and in 60% after 6 hours, and submucosal bleeding was manifest 2 hours after inoculation of endotoxin in 40% and in 20% after 6 hours. But both submucosal edema and bleeding were not observed 24 and 72 hours after inoculation of endotoxin.
2 Mixture of E. coli (06) 10³ cells/ml and endotoxin (10μg/ml or 1mg/ml) were inoculated into urinary bladder. Inoculation of mixture of E. coli (06) and endotoxin caused more marked disruption of the mucin after 6 and 24 hours after than did the inoculation of E. coli 10³ cells/ml alone. Disappearance of mucin, submucosal edema and bacterial invasion wereobserved more markedly in cases of mixture of endotoxin 1mg/ml than in cases of mixture of endotoxin 10μg/ml.