STUDY OF IN VITRO EFFECT OF MPA ON ESTABLISHED CELL LINE FROM HUMAN RENAL CELL CARCINOMA

Abstract

In vitro effect of MPA (medroxy progesterone acetate) on the established cell line from human renal cell carcinoma (NUR) was examined by observing growth curve, incorporation of ³H-thymidine and DNA histogram of tumor cells. As controls, human bladder cancer cell line (NUB), human testicular cancer cell line (NUT), normal human kidney cell (NHK) and BHK cell were examined. The results obtained were as follows.
1) Effect of MPA on growth curve.
The growth of NUR cells was suppressed as compared to control by MPA at concentration of more than 0.1μg/ml, but other cell lines were not suppressed at concentration of less than 1.0μg/ml MPA.
2) Effect of MPA on incorporation of ³H-thymidine.
Significant suppression was observed in NUR as compared to other cell lines. At concentration of 10μg/ml MPA, % inhibition was 52% to NUR, 20% to NUB, 19% to NUT and 6% to NHK.
3) Analysis of DNA histograms of tumor cells.
The G₀₊₁ phase fraction increased and S+G₂M phase fraction decreased in NUR by exposure to MPA for 72hrs. But in other cell lines, there appears to be no significant changes in DNA histograms.