IN VITRO CHEMOSENSITIVITY TESTING OF HUMAN UROGENITAL CARCINOMAS BY CLONOGENIC ASSAY

Abstract

Different type of human urogenital carcinomas were tested for their in vitro clonogenicity and chemosensitivity using a human tumor clonogenic assay (clonogenic assay) originally described by Salmon and his colleagues.
Sixty one different samples of human urogenital carcinomas, including 25 renal cell, 25 uroepithelial, 2 prostatic, 3 penile and 6 testicular carcinomas were tested. Clonal growth (≥5 colonies per plate) occurred in 42 of the 61 specimens (69%), including 37 of 53 solid tumors obtained from primary site, 2 of 2 metastatic lymph nodes, 2 of 2 pleural effusions and 1 of 3 bladder burbotages. However, an adequate growth for chemosensitivity testing (≥30 colonies per control plate) occurred in only 32 of the 61 specimens (52%), including 17 of 25 renal cell, 9 of 25 uroepithelial, 1 of 2 prostatic, 2 of 3 penile and 3 of 6 testicular carcinomas. Notably, the highest clonogenicity was showen in renal cell carcinomas.
A greater than 70% reduction in colony formation was obtained in 1/27 (3.7%) of anticancer drugs tested for renal cell carcinomas and 1/46 (2.2%) for uroepithelial carcinomas, when in vitro chemosensitivity testing was performed with a one-hour exposure of tumor cells to the drugs at a eoncentration 1/10 to 1/20 of that achievable in patient's palsma levels after a standard clinical dose.