1986 Volume 77 Issue 4 Pages 653-658
A new cell line (YCR-1) was established from a renal cell carcinoma of a 62-year-old woman. Primary cells were seeded in soft agar medium. Formed colonies in agar were transferred to liquid culture after 2 weeks and have been maintained for 19 months. They are now over 100 transfer generations. The cells are epithelioid morphology and free of contact inhibition in liquid culture. Their doubling time in culture was 26.5 hours. The chromosome number of the cells were hyperdiploid.
Heterotransplantations of the cells into the back of nude mice produced tumors that were histologically akin to the original tumor. Electron microscopic study revealed abundunt microvilli on their cellular surface and the polarity in the arrangement of cellular organelles.
