IN VITRO CULTIVATION OF HUMAN TESTICULAR GERM CELL TUMORS
(I) Establishment of Three Cell Lines Derived from Embryonal Carcinomas
1986 Volume 77 Issue 5 Pages 772-779
Details
1986 Volume 77 Issue 5 Pages 772-779
Between April 1977 and September 1982, monolayer cultures of 33 human testicular germ cell tumors (16 seminomas and 17 non-seminomatous germ cell tumors) were attempted and three embryonal carcinomas were established as proliferating long-term culture, but long-term culture of seminomas was not successful. These were designated as NEC8, NEC14 and NEC15, respectively.
The characteristics of these three cell lines and a cell line, ITO (embryonal carcinoma) were investigated. Morphologically, they are alike in character. Chromosome studies revealed that these cell lines have numerical and structural change involving chromosome 1. The involvement of chromosome 1 in aberrations may be associated with malignant nature of embryonal carcinomas.
