1986 Volume 77 Issue 6 Pages 886-895
A fluorescence study was performed on specimens from 20 primary and 2 metastatic sites and tumors themselves of TSU-R1, our new cell line derived from renal cell carcinoma, grown in nude mice using 3 lectins, soybean agglutinin (SBA), peanut agglutinin (PNA), and wheat germ agglutinin (WGA), and antibodies to renal tubular antigen (RTA).
Anti-RTA reacted with carcinoma cells in all specimens of primary tumors, while SBA reacted in 9 (45%), PNA in 3 (15%) and WGA in 11(55%). In carcinoma cells from metastatic sites, only SBA did not react with them. Although we observed the presence of RTA in the tumor of TSU-R1 transplanted to a nude mouse at the 10th passage, RTA could not be found in the tumor transplanted at thr 50th passage. WGA reacted with the carcinoma cells in both passages of the tumors of TSU-R1, but SBA and PNA did not bind to either of them.
This fluorescence study seems to be a simple and useful method to investigate the characteristics of renal cell carcinoma. Since changes of glycocompounds may correlated to malignant potential of the carcinoma cells, further study with lectins will be necessary.
