1986 Volume 77 Issue 8 Pages 1324-1330
Kinetics of hematoporphyrin diacetate (Hp-diAc) and hematoporphyrin disilate (Hp-diSi), which were synthesized as single chemical compounds, were investigated in rats with subcutaneously transplanted AH-109A hepatocellular tumor. Hematoporphyrin derivative (Hp-D), a complex mixture of several porphyrins, was used as the control. The agents were given intravenously at a dose of 10mg/kg; tissue specimens obtained as predetermined intervals were submitted to fluorescent microscopic observation and porphyrin concentration in the tissues was assessed with a fluorescent spectrophotometer.
Hp-diAc, Hp-diSi and Hp-D showed, in general, a trend of tissue distribution in the following order: «liver, spleen»tumor, kidney, lung>testis, muscle. Hp-diAc and Hp-D levels in these tissues gradually decreased, while Hp-diSi was retained for a longer period of time up to 96 hours after injection. Tumor surrounding muscle ratio of porphyrin concentration (T/M value) ranged from 2.7 to 7.5 for Hp-D and from 3.7 to 6.6 for Hp-diSi, indicating a high intratumorous accumulation of these two substances. On the other hand, T/M value of Hp-diAc remained at a low level ranging from 1.0 to 1.7.
The present study suggests the possible usefulness of Hp-diSi as a porphyrin with tumor affinity.
