TOTAL LDH ACTIVITIES AND LDH ISOZYME PATERNS IN THE TISSUES OF TESTICULAR TUMORS

Abstract

The total LDH activities and LDH isozymes were measured in 6 normal testicular tissues and 11 seminoma tissues. The total LDH activities of seminoma tissues were 3778.4±3674.7W.U./mg protein (mean±S. D.), significanlty higher than those of mormal testicular tissues (787.2±502.4W.U./mg protein) (p<0.05). In normal testicular tissues, 5 isozymes (from LDH1 to 5) and LDHX were detected, but in semimona tissues only 5 isozymes were detected and LDHX was not detected. In semmoma tissues, compared with normal tissues, LDH1 and LDH2 were elevated. Since it is widely accepted that LDHX appears in primary spermatocytes, these data suggest that the cells of semimoma are composed of less differentiated cells than spermatogonia.