AN EXPERIMENTAL STUDY OF WHOLE BLADDER WALL PHOTODYNAMIC THERAPY USING A LIGHT-SCATTERING MEDIUM
A Preliminary Report
1988 Volume 79 Issue 5 Pages 807-813
Details
1988 Volume 79 Issue 5 Pages 807-813
Laser light scattering effect of Intralipos were studied with a glass flask instead of the bladder and with an argon-dye laser. Red light was irradiated from the tip of a 400μm quartz fiber kept at the center of the flask containing 200ml of 0.5% Intralipos solution with a wavelength of 630nm and the output of 500mW. Homogeneous laser light scattering was most efficiently obtained with an average power density of 0.77±0.07mW/cm2. Hematoporphyrin derivative (HpD) fluorescence intensity of frozen sections of Vχ2 carcinoma transplanted into the bladder neck of rabbits 48hr after injection was 20% greater than that of the normal bladder wall sections. In whole bladder wall photodynamic therapy (PDT) study of the carcinoma-bearing bladder 48hr after 0.5mg/kg body weight HpD i.v. injection, a light energy of 100Joules/cm2 was used and definite necrotic changes mixed with vacuolar degeneration and pyknosis of the tumor cells were found. These results suggested the usefulness of 0.5% Intralipos as a scattering medium for whole bladder wall PDT.
