Abstract
Epithelioid cells that had grown in short-term cultures derived from 10 cases of adenocarcinoma (PCa) and 10 cases of hyperplasia (BPH) of the prostate were karyotyped by the G-banding method for the pathogenesis of these disease. PCa specimens included 4 well, 2 moderately, and 4 poorly defferentiated types, and were obtained by perineal needle biopsy from 4 patients in stage B and 6 patients in stage D2. Cells liberated from metastatic lymph node lesions of 2 patients with poorly defferentiated PCa were also analyzed directly without cultivation in vitro. All BPH specimens were obatained by prostatectomy, and cells that had grown in epithelioid pattern in short-term cultures were analyzed. In PCa, hyperploidy was seen in all but 2 cases. Structure analysis disclosed abnormality of chromosome 16 in 4PCa, delection of Y in 3PCa, abnormality of chromosomes 7, 14, 15, 18, and 19 in 2PCa, and abnormality of chromosomes 3, 4, 17, and 21 in 1PCa. Multiple markers were observed in 1 patient, and hyperploidy in another patient with metastatic lymph nodes. All but 2 cases of BPH were diploid. Normal male karyotypes were seen in 6BPH. Trisomy of chromosomes 7 and 16 were observed in 2BPH. Of 4 patients with stage B PCa, 3 who have been alive for 3 years to date had multiple abnormalities, whereas 1 patient who died 2 years after diagnosis had few abnormalities. Of 8 patients with stage D2PCa, 2 patients who have been alive for 3 years to date had no or few abnormalities, while 6 patients who died 1.5-3 years after diagnosis had multiple abnormalities. These findings indicate that chromosomal abnormalities may be seen in many PCa but not in BPH, and that PCa with many abnormalities may be more malignant than PCa with few abnormalities.
