EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF PROSTAGLANDIN I₂ DERIVATIVE

(OP-41483) ON DAMAGE OF WARM ISCHEMIC KIDNEY

Abstract

The protective effect of prostaglandin I₂ derivative (OP-41483), which has actions of antiaggregation of platelets and vasodilatation, to damage by warm ischemia was investigated in rabbit kindeys.
Experiment 1) Two weeks after the right nephrectomy, the left renal artery and vein were temporarily clamped for 180 minutes. Before and/or after the clamp was released, OP-41483 was given by i. v. drip (100-1, 000ng/kg/min) for 10 minutes. Renal blood flow (cortical flow and medullary flow) was measured before and after warm ischemia. Changes of blood urea nitrogen and serum creatinin level were measured after warm ischemia. Experiment 2) Bilateral renal arteries and veins were clamped for 180 minutes. OP-41483 (300ng/kg/min) was administered before and/or after clamping the aforementined vessels. The left kidney was removed after warm ischemia of 180 minutes, the other one was removed 60 minutes after the clamp was released, and histological examination was studied.
Results were as follows: The blood urea nitrogen and serum creatinin level of OP-41483 treated group were lower than that of non-treated group. The best protective effect was seen when 1, 000ng/kg/min OP-41483 was administered before and after the ischemia. No significant decrease of the renal blood flow was demonstrated after the warm ischemia in the OP-41483 treated group. Histopathologically, OP-41483 has the inhibit effect on detachment of tubular cell from the basal lamina, Bowman's space exudate and swelling of mitochondria.