ACTIVE UPTAKE OF OXALATE IN A RENAL TUBULAR CELL LINE (LLC-PK₁)

Abstract

The oxalate uptake was studied in LLC-PK₁ cells, an epithelial cell line originated in proximal tubular cells of porcine kidney. It was clear that the cells contain an oxalate exchanger highly sensitive to 4, 4-diisothio-cyanostilbene-2-′ disulfonic acid (DIDS). The uptake was inhibited by the addition of inorganic anions (chloride, sulphate and bicarbonate) to the reaction system, but was unaffected by sodium ion. The data suggest the possibility that it should be consistent with at least two transport systems for oxalate in LLC-PK₁ cells, a SO₄^--/oxalate/HCO₃^- exchanger and a Cl^-/oxalate exchanger.