HISTOCHEMICAL EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) FOR PRE AND POST CHEMOTHERAPEUTIC BLADDER CANCER

Abstract

We examined whether the staining of proliferating cell nuclear antigen (PCNA) could be useful as a marker of chemotherapeutic effectiveness in nine patients with invasive bladder cancer treated by bilateral internal iliac artery infusion of cisplatin (CDDP). Two types of monoclonal antibodies PC-10, 19A2 were used for immunohistochemical staining in formalin-fixed, paraffin-embedded tissue sections. There was no difference between positive rates of PC-10 and 19A2.
The mean positive rates between pre-chemotherapeutic specimens (15.4±6.7%) and post-chemotherapeutic specimens (4.2±3.1%) showed statistically significant difference (p<001). The immunohistochemical evaluation of PCNA has balue for investigation of cell's turnover. Therefore, the changes of PCNA-positive rate could be a historagical parameter for the evaluation of chemotherapeutic effects.