2010 Volume 31 Issue 2 Pages 39-47
Full-field optical coherence tomography (FF-OCT) is a non-scanning horizontal cross-sectional imaging technique based on interference microscopy. By employing a high-definition microscopic imaging system and a low-coherence interferometer, we have achieved sub-cellular level biological tissue imaging. The majority of conventional FF-OCT imaging has involved the ex vivo imaging of rat or porcine retinas, and high-resolution retinal images have been demonstrated. However, in vivo retinal imaging has not been realized, due to slow measurement speed. We have built a high-speed imaging system and have applied the new FF-OCT system to anterior segment imaging of the rat in vivo. Feasibility studies of FF-OCT human retinal imaging were then performed. Visualization of retinal nerve fiber bundles is demonstrated for the first time, to the best of our knowledge.
