Mechanical stromal vascular fraction for peripheral nerve disorder: Current status and future perspectives

Abstract

　Adipose-derived stem cells (ADSCs) have been reported to promote peripheral nerve regeneration through multiple mechanisms, including the secretion of neurotrophic factors, enhancement of angiogenesis, and anti-inflammatory effects. We previously reported that supplementing nerve reconstruction procedures with ADSCs improves regenerative outcomes in peripheral nerve defects. In contrast, mechanical stromal vascular fraction (mSVF) , which is obtained from adipose tissue through mechanical processing without the use of enzymatic digestion, is considered advantageous for clinical application because it enables the rapid and low-cost isolation of heterogeneous cell populations that include ADSCs. However, mSVF still presents challenges, such as variability in cell composition depending on the extraction method and insufficient understanding of the underlying mechanisms. Further investigation is required to advance mSVF toward clinical use. In this review, we summarize current research on SVF in peripheral nerve regeneration and discuss the present status and future prospects.