Targeted Proteomics: Current Technical Advance and Sample Preparation

Abstract

Advances in sensitivity, scanning speed, and resolution of mass spectrometry extensively facilitated comprehensive identification and quantification of proteome. However, one of the major techniques used in the current proteomics, data-dependent acquisition (DDA), has technical limitations that prevent in-depth proteome analysis across large number of samples. Recently, targeted proteomics, such as multiple-reaction monitoring (MRM) has been developed to promote high-throughput, accurate and consistent quantification of given protein sets, that serves the extended application of proteomics for biology. In this review, I describe the current topics in targeted proteomics and the sample processing procedure required for targeted proteomics.