A Practical Introduction to Targeted Proteomics

Abstract

Targeted proteome analysis is an approach for quantitative analysis of multiple target peptides using liquid chromatography-triple quadrupole mass spectrometry. Although it is essentially similar to that of quantitative analysis of small molecules, several specific techniques are required for targeted proteome analysis including sample preparation methods, handling of nano-LC, and construction of SRM assay methods. Recent progresses in the sample preparation protocols, improved stability of modern nanoLC machines, and development of softwares enable a wide application of the targeted proteome analysis in biological studies.