Quantification of Extracellular Vesicle Proteins by Targeted Proteomics and Search for Novel Colorectal Cancer Markers

Abstract

Early diagnosis of cancer by liquid biopsy is desired to be realized in cancer treatment. Large-scale proteomic analysis for biomarker discovery has been actively conducted. However, biomarkers that can be used for early diagnosis have not been developed. In recent years, extracellular vesicles have attracted attention as biomarker resources for liquid biopsy. In our laboratory, extracellular vesicles were extracted from colon cancer patient serum and biomarker candidate proteins were identified by shotgun proteomics. These candidate proteins were further verified by targeted proteomics, SRM. In this article, we describe the pretreatment method of serum samples and optimization of analysis method in SRM.