2021 Volume 6 Issue 1 Pages 1-7
Mass spectrometry and many kinds of related techniques have made a big contribution to proteomic research. On the other hand, various types of approaches other than mass spectrometry have also been used in proteomic analyses, making the best use of their characters and advantages. Large-scale data are vital for efficient finding of molecular functions and mechanisms in life. In addition, it is also significant to understand living organism as a system where whole molecules, such as proteome, coordinate or compete with one other to drive biological activity. In this review, I will introduce a variety of proteomic approaches independent of mass spectrometry, especially for budding yeast, and describe my perspective about what is required to further expand the proteomic research.
