Development of Basic Proteomics Technology Using Monolithic Silica Columns and Its Application to Analysis of Pluripotent Stem Cells

Abstract

Current proteome analysis method is an effective method to comprehensively analyze the proteins, but it is still difficult to identify and quantify the proteins of a specific cell in a single analysis due to the complexity of the proteome samples. We have been working on the development of basic technologies to solve this problem and improve the identification and quantification efficiency. We have focused on the use of monolithic columns to improve separation efficiency and have developed the RiMS (removal of interference mixture spectra) method, which improves the accuracy of isobaric labelling quantification. We applied this method to the analysis of pluripotent stem cells, resulting improved quantification accuracy of cell type-specific proteins. In this paper, we will introduce the RiMS method with an overview of the methods used for quantitative proteome analysis.