Proteome Letters
Online ISSN : 2432-2776
ISSN-L : 2432-2776
Is Protein Solubilization Necessary for Proteomics Sample Preparation?
Atsushi HatanoMasaki Matsumoto
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JOURNAL FREE ACCESS

2023 Volume 8 Issue 1 Pages 13-17

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Abstract

In bottom-up proteomics, sample preparation process, particularly protein digestion, has a significant impact on protein identification and quantification. Although several methods for protein digestion have been developed, it is generally believed that complete protein solubilization with detergent is a critical step for efficient enzymatic digestion. However, since detergent affects subsequent sample preparation and liquid chromatography-mass spectrometry (LC-MS) analysis, they must be completely removed from protein and peptide solution. Especially in multi-sample or very low-quantity sample processing, detergent removal can be a challenge as it can cause sample loss and decrease sample preparation efficiency. Recently, several detergent-free enzymatic digestion methods with incomplete protein solubilization have been reported, and their digestion efficiencies were comparable to that of the conventional method using detergent. In this review, we introduce recently developed methods of enzymatic digestion of proteins without solubilization.

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© 2023 Japanese Proteomics Society
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