2023 Volume 8 Issue 2 Pages 35-42
Targeted proteomics is a technique in which target proteins are quantified using mass spectrometry, and it has several advantages over non-targeted proteomics regarding sensitivity, quantitation, and throughput. Targeted proteomics is a technology that complements non-targeted proteomics by accurately quantifying candidate proteins identified by quantitative proteomics. Furthermore, it has advantages over antibody-based protein quantification regarding high specificity and multimolecular quantification, making it effective for quantifying proteins for which no antibodies are available. Because of these characteristics, it is used to validate candidate biomarker proteins and quantify proteins with post-translational modifications. However, targeted proteomics requires knowledge that is different from that required for non-targeted proteomics. In this article, we summarize the technical basics of targeted proteomics, including its technical outline and expertise, and discuss our achievements in the application of targeted proteomics.
