2024 Volume 9 Issue 1 Pages 1-11
Because cancer has unique plasticity and diversity in its metabolic state, systematic interpretation using multiple omics data is important. Metabolomics requires analytical tools that can accurately and comprehensively capture quantitative changes in metabolites inside and outside of cancer cells. However, most critical metabolites, such as amino acids and organic acids, are difficult to quantify by LC-MS using common reversed-phase analytical columns owing to their polarity and sensitivity limitations. To address this issue, we developed a system for the simultaneous absolute determination of amino acids, ATP, and NAD using a column packed with polymer particles featuring mixed amino group modifications. Furthermore, we adapted an organic acid measurement system using the 2-picolylamine derivatization method, and an adamantylethyl group column was used to establish a system for the absolute quantification of major cancer metabolomes using an isotope dilution method. Application of this method to three prostate cancer cell lines, combined with data-independent acquisition (DIA) proteomic data, revealed cell line-specific metabolic transitions. These methods introduced in this report are expected to be an important analytical platform for cancer metabolomics.
