2003 Volume 13 Issue 1-2 Pages 15-22
To develop a vaccine against cryptosporidiosis in cattle, we constructed a recombinant vaccina virus expressing an immunodominant surface protein p23 of Cryptosporidium parvum (C. parvum) sporozoites. Antibody against p23 recognized the p23 expressed in RK13 cells infected with the recombinant vaccinia virus as an approximately 23 kDa specific band in Western blotting analysis. The immunization of Balb/c mice with the recombinant induced the production of immunoglobulin G1 (IgG1). However, the level of immunoglobulin G2a (IgG2a) production was very low. These results indicate that the p23 expressed by the recombinant vacinia virus induced predominantly a Th2 response in Balb/c mice. In the case of C57BL mice, delayed type hyper sensitivity was observed. However, the induction of antibody production against p23 was not detected.
