Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for Vaccine against African Trypanosomosis

Kuboki Noritaka.; Yokoyama Naoaki.; Namangala, B.; Okamura Masashi.; Inoue Noboru.; Takagi Hideaki.; Nakayama Tomoko.; Nishikawa Yoshifumi.; Ikehara Yuzuru.; Kojima Naoya.

doi:10.32268/jprotozoolres.18.1_1

Kuboki Noritaka., Yokoyama Naoaki. , Namangala, B., Okamura Masashi., Inoue Noboru., Takagi Hideaki., Nakayama Tomoko., Nishikawa Yoshifumi., Ikehara Yuzuru., Kojima Naoya.

Author information

Kuboki Noritaka.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Yokoyama Naoaki. Corresponding author
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Namangala, B.
The University of Zambia, Faculty of Veterinary Medicine, Department of Paraclinical Studies, Box 32379, Lusaka, Zambia
Okamura Masashi.
Laboratory of Zoonosis, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan
Inoue Noboru.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Takagi Hideaki.
The Institute of Glycotechnology, The Department of Applied Biochemistry, Tokai University Hiratsuka, Kanagawa 259-1292, Japan
Nakayama Tomoko.
The Institute of Glycotechnology, The Department of Applied Biochemistry, Tokai University Hiratsuka, Kanagawa 259-1292, Japan
Nishikawa Yoshifumi.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Ikehara Yuzuru.
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, lbaraki 305-8568, Japan
Kojima Naoya.
The Institute of Glycotechnology, The Department of Applied Biochemistry, Tokai University Hiratsuka, Kanagawa 259-1292, Japan

JOURNAL FREE ACCESS

2008 Volume 18 Issue 1 Pages 1-10

DOI https://doi.org/10.32268/jprotozoolres.18.1_1

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Abstract

Since an oligomannose-coated liposome (OML) vaccine strategy has been shown to induce a T helper-type 1 (Th1) immune response, it might be a suitable method to control the early stage of African trypanosomosis. In order to evaluate the adjuvant effect of OML, the present study was conducted to investigate the effect of bovine serum albumin (BSA)-encapsulating OML (BSA + OML) on host immunity and the Trypanosoma brucei gambiense infection in mice. Immunization with the BSA + OML induced strong ex vivo proliferation of lymphocytes, and the cultured cells notably produced interleukin (IL)-2, interferon (IFN)-γ, tumor necrosis factor (TNF）-α,IL-6, IL-10, IL-4, and IL-5. The sera from the mice immunized with BSA + OML displayed the similar cytokines profile, but, in addition, they contained IL-12p70. Following T. b. gambiense infection, the BSA + OML group exhibited a significantly reduced number of the parasites, slightly delayed survivals, and the reduction of IL-10 in the sera as compared to the other groups, possibly due to a skewed Th1 immune response. Therefore, the OML-based vaccine described here could provide a platform to apply for various protozoan diseases susceptible to Th1-immunity as a vaccine adjuvant.

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