Genomic Analysis of Sarcocystis by Contour-clamped Homogeneous Electric Fields

Abstract

The chromosomal DNA of Sarcocystis gigantea and Sarcocystis muris were digested with NotI and SfiI and characterized in contour-clamped homogeneous electric fields. Under these electric fields, the chromosomal DNA of S. gigantea and S. muris showed different electrophoretic mobilities. Therefore, it appears that this technique may be useful in future studies on the differentiation of Sarcocystis species. The results of NotI and SfiI digestion of S. muris chromosomes suggested that there is an absence of a significant number of recognition sites for these two enzymes in the genome of S. muris. The addition of the sizes of 15 SfiI fragments of S. gigantea DNA suggested that the S. gigantea genome is larger than 14 mb.