Detection of Trypanosoma evansi infection among parasitologically and immunologically negative animals by polymerase chain reaction

Abstract

A study was undertaken to detect Trypanosoma evansi infection by polymerase chain reaction (PCR) among clinically ill cattle, buffaloes and horses which were suspected for 'surra', but found negative by parasitological and immunological tests. Detection of trypanosomal DNA based on PCR have been found more useful tool for diagnosis of the infection in clinically ill animals which were declared as negative by examination of Giemsa stained blood smears, mouse inoculation test and antibody detection enzyme linked immunosorbent assay (ELISA) test.