Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

Nakamura, C.; Hayashi, K.; Mohamad, A. T.; Masatani, T.; Zhang, G.; Jia, H.; Kumagai, A.; Zhang, H.; Zhou, J.; Nishikawa, Y.; Sugimoto, C.; Xuan, X.

doi:10.32268/jprotozoolres.23.1-2_7

Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

Nakamura, C., Hayashi, K., Mohamad, A. T., Masatani, T., Zhang, G., Jia, H., Kumagai, A., Zhang, H., Zhou, J., Nishikawa, Y., Sugimoto, C., Xuan, X.

Author information

Nakamura, C.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Hayashi, K.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Mohamad, A. T.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Masatani, T.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Zhang, G.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Jia, H.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Kumagai, A.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Zhang, H.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Zhou, J.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Nishikawa, Y.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]
Sugimoto, C.
Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan [Japan]
Xuan, X. Corresponding author
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan [Japan]

JOURNAL FREE ACCESS

2013 Volume 23 Issue 1-2 Pages 7-13

DOI https://doi.org/10.32268/jprotozoolres.23.1-2_7

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Abstract

Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band corresponding to a molecular mass of 80 kDa, which was similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/BgTRAP-infected RK13 cells. Moreover, mice inoculated with VV/BgTRAP produced antibody that specifically reacted with the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

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