Comparison of the relative tolerance of Colpoda resting cysts and vegetative cells to electrostatic exposure

Takeru Saito; Kazuma Yabuki; Yuta Saito; Hiroki Yamanobe; Ryota Saito; Shuntaro Hakozaki; Hitoshi Amano; Yohey Ogawa; Yoichro Sogame

doi:10.32268/jprotozoolres.33.Vol.1-2_32

Comparison of the relative tolerance of Colpoda resting cysts and vegetative cells to electrostatic exposure

Takeru Saito, Kazuma Yabuki, Yuta Saito, Hiroki Yamanobe, Ryota Saito, Shuntaro Hakozaki, Hitoshi Amano, Yohey Ogawa, Yoichro Sogame

Author information

Takeru Saito
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Kazuma Yabuki
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Yuta Saito
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Hiroki Yamanobe
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Ryota Saito
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Present address: Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan.
Shuntaro Hakozaki
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Hitoshi Amano
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
Yohey Ogawa
Department of Pathology and Immunology, Washington University, School of Medicine, Missouri, 63110-1093, USA.
Yoichro Sogame Corresponding author
National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.

JOURNAL FREE ACCESS

2023 Volume 33 Issue Vol.1-2 Pages 32-42

DOI https://doi.org/10.32268/jprotozoolres.33.Vol.1-2_32

Details

Abstract

The soil protist ciliate Colpoda cucullus forms resting cysts that have extreme tolerance to many environmental stresses. In this study, we show that Colpoda resting cysts, unlike vegetative cells, can survive electrostatic exposure. The viability of vegetative cells fell with the number of electrostatic exposures; cyst viability did not show this response. The morphology of exposed cysts appeared to be essentially normal at the optical microscope level; excysted cells from exposed cysts retained a similar proliferative ability to those of non-exposed cysts, whereas most vegetative cells were fatally damaged by electrostatic exposure.

Corresponding author

Register with J-STAGE for free!