The Journal of Protozoology Research
Online ISSN : 2434-7027
Print ISSN : 0917-4427
Continuous in vitro Cultivation of Babesia ovata
IKUO IGARASHIAVGAANDORJIIN AVARZEDTETSUYA TANAKANOBORU INOUEMASANARI ITOYOSHITAKA OMATAATSUSHI SAITONAOYOSHI SUZUKI
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JOURNALS OPEN ACCESS

1994 Volume 4 Issue 3 Pages 111-118

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Abstract

Basic method for in vitro cultivation of Babesia ovata was examined using a method which was developed by Vega et al. for cultivation of B. bigemina, a closely related organism. The parasites obtained from an infected SCID mouse were initiated their multiplication within adult bovine RBC in Medium 199 supplemented with 40% adult bovine serum under a low oxygen atmosphere, 5% O2, 5% CO2 and 90% N2. Although no proliferation of B. ovata maintained in 5% CO2 in air was seen during the initial 5 days, the parasites passaged three times under the low oxygen atmosphere were readily cultured in 5% CO2 in air, as well as under the low oxygen atmosphere. The parasites were propagated in the RBC stored in Vega y Martinez solution at 4℃ for up to 2 months. Babesia ovata-infected RBC from cultures were successfully cryopreserved in 10% polyvinylpyrrolidone in Vega y Martinez solution and used to initiate new culture not only under the low oxygen atmosphere but also in 5% CO2 in air.

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© 1994 National Research Center for Protozoan Diseases, National University Corporation Obihiro University of Agriculture and Veterinary Medicine
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