1996 Volume 6 Issue 3 Pages 83-93
We have sequenced the cDNA encoding the major surface antigens P30 and P22, and the excreted-secreted major antigen P23 of the virulent RH strain and avirulent Beverley and S-273 strains of Toxoplasma gondii by direct PCR. The amplified sequences of antigen cDNA were compared. Complete homology was found in the cDNAs encoding the P22 antigen in the RH and S-273 strains of T. gondii, and high homology but not complete was found between the cDNA encoding P23 and P30 in the Beverley and S-273. An interesting finding was the amino acid sequence differences relating to surface charge of P30 of virulent and avirulent strains of T. gondii. These phenomena might be associated with infectious difference in cells and/or the differences in pathogenicity.