Picosecond Spectroscopy on Reverse Photoreaction from Batho-Intermediate of Bacteriorhodopsin at 6.5 K

Abstract

The reverse photoreaction from batho-intermediate of bacteriorhodopsin has been investigated by picosecond laser flash photolysis at 6.5 K. Upon exciting batho-intermediate with 694 nm light pulse of 31±4 ps width, the increase of absorbance around 570 nm and concomitant decrease of that around 630 nm are observed without delay time. Based on the above result, the models for the primary photochemical reaction of bacteriorhodopsin are discussed.