Host: The 92nd Annual Meeting of the Japanese Pharmacological Society
Name : The 92nd Annual Meeting of the Japanese Pharmacological Society
Number : 92
Location : Osaka
Date : March 14, 2019 - March 16, 2019
Primary cilium is a nonmotile sensory organelle that possesses selective membrane receptors. The cilium is dynamically regulated in a cell cycle-dependent manner; it is displayed at the G0/G1 phase of many cell types, including neural progenitor cells, and resorbed prior to the S phase. The ciliary dynamics has pivotal roles in development of many tissues/organs. However, the molecular mechanism how the cilium controls cell proliferation remains largely unknown. We found that IGF-1 was one of the growth factors that promoted proliferation of neural progenitors. Tctex-1, a light chain of cytoplasmic dynein that plays a key role in ciliary resorption, can be free from dynein complex when it is phosphorylated at Thr 94. We also found that phospho-Tctex-1 was enriched at the ciliary base in the cells. Molecular approaches analyzed in an immortalized retinal pigment epithelial cell line revealed the physical and functional interaction of phospho-Tctex-1 with the regulators of actin dynamics such as annexin A2, Arp2/3 complex and Cdc42, which promoted branched actin polymerization and dynamin- and clathrin-dependent endocytosis at peri-ciliary region. Our study demonstrated that these mechanisms collectively regulate ciliary resorption and proliferation of neural progenitors.
