Host: The 92nd Annual Meeting of the Japanese Pharmacological Society
Name : The 92nd Annual Meeting of the Japanese Pharmacological Society
Number : 92
Location : Osaka
Date : March 14, 2019 - March 16, 2019
Recent advances in super-resolution microscopy have overcome a limitation ofspatial resolution in fluorescence imaging imposed by the diffraction of light,enabling nanoscale molecular imaging with conventional fluorescent probes.Single-molecule localization based super-resolution microscopy, includingstochastic optical reconstruction microscopy (STORM), provides multi-color andthree-dimensional imaging with a remarkable spatial resolution, and thusresolves detailed nanostructures in biological specimens. Fortunately, it can beperformed with a simple wide-field microscope setup and is completely compatiblewith immunohistochemical fluorescent staining methods. In this seminar, I willtalk a detailed method for STORM imaging, from sample preparation to imageanalysis, with some tips on how to improve the spatial resolution. I will thenshow some examples for STORM imaging of immunohistochemical specimens, includingthe brain and pancreas.
